Brainbow 32

I am trying to image brainbow 32 transgenic mice but seem to get a lot of spectral overlap. The ten purkinje cells in this field are labeled by at least seven distinct colors antibody amplified and numbered ivii.

A Rainbow Reporter Tracks Single Cells And Reveals Heterogeneous

A brainbow 32 strain with enhanced fluorescent protein expression is available as stock tgthy1 brainbow327jrsj 021227.

Brainbow 32. 1ab one xfp is expressed by default in cre negative cells. Brainbow is not just for brains anymore. Brainbow labeling has also been applied to cell marking and lineage tracing in other systems such as zebrafish and drosophila.

Also known asbrainbow 32 line 7 these brainbow 32 founder line 7 mice allow labeling of individual neuronal types including neurons of spinal cord cortex hippocampus cerebellum and retina with approximately 90 distinguishable color variations in cre recombined cells. Design of brainbow 31 and 32 transgenes in brainbow 1 2 and 30 fig. Because cre is selectively expressed by purkinje cells in the l7 cre line no other cell types are labeled.

Brainbow 31 line 3 l7 cre mouse. Design of brainbow 31 and 32 transgenes. The presence of a default xfp has both drawbacks and.

The presence of a default xfp has both drawbacks and advantages. In brainbow 1 2 and 30 and ref. 15 one xfp is expressed by default in cre negative cells.

F cerebellum from a brainbow 32 line 7 caggs creer. The technology has been adapted for general use anywhere in the mouse. I want try a linear unmixing protocol which requires reference images of the fluorophores.

In cases of limited cre expression this xfp is expressed in a majority of cells reducing spectral diversity among recombined neurons. Brainbow 1 and 2 have been further refined to create brainbow 32. A more recent version brainbow 32 permanently barcodes neurons with fluorescent proteins that are all targeted to the plasma membrane thereby facilitating lineage tracing delineation of.

Multicolor Animals Using Fluorescent Proteins To Understand